Micro Alkali Proteinase Assay Kit
Specification : 100 tubes/48 samples, 50 tubes/24 samples
Test Method : Trace Method / Visible Spectrophotometry
Meaning of Measurement:
AKP is an enzyme that catalyzes the hydrolysis of protein peptide bonds under alkaline conditions and is a serine protease. AKP is a serine protease that hydrolyzes ester and amide bonds, and has transesterification and transpeptidation functions. The enzyme is one of the main industrial enzymes, widely used in pharmaceutical, silk, food, tanning and other industries.
Measurement principle:
Under alkaline conditions, AKP hydrolyzes casein to produce tyrosine; under alkaline conditions, tyrosine reduces phosphomolybdic acid to produce tungsten blue; tungsten blue has a characteristic absorption peak at 680nm, and the rate of increase in absorbance at 680nm is measured to calculate AKP activity.
Prepared Instruments and Supplies:
Micro Alkali Proteinase Assay Kit
Visible spectrophotometer/enzymometer, micro glass cuvette/96-well plate, water bath, magnetic stirrer, adjustable pipette gun, 0.5 mL EP tube and distilled water.
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