Method:Micro method
Specification: 100 tubes/96S
Storage conditions: low temperature and light protection
Validity: 6 months
Note: Choose 2-3 samples with large expected differences for pre-calibration before formal measurement.
Measurement significance:
Blood phosphorus mainly refers to inorganic phosphorus in blood, in the form of inorganic phosphorus salts. Calcium and phosphorus concentrations in plasma are closely related, and when expressed in mg/dL, the product of the two ([Ca] × [P]) is 30 to 40. When ([Ca] × [P]) > 40, calcium and phosphorus are deposited in bone tissue in the form of bone salts; if ([Ca] × [P]) < 35, it will impede bone calcification, and even dissolve bone salts, which will affect osteogenesis. The relative stability of blood calcium and phosphorus levels depends on the relative balance of calcium and phosphorus absorption and excretion and the metabolism of calcification and decalcification. This balance is regulated by hormones such as vitamin D3, parathyroid hormone and calcitonin.
Principle of Measurement:
After removing the organic phosphorus in serum, the inorganic phosphorus salt and ammonium molybdate reagent generate phosphomolybdic acid, which is reduced by ferrous sulphate and turns blue, with light absorption at 620 nm; the phosphorus content of blood is calculated by measuring the absorbance at 620 nm.
Bring your own instruments and supplies:
Centrifuge, adjustable pipette gun, visible spectrophotometer/enzymometer, microquartz cuvette/96-well plate, and distilled water.
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